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Files in this Data Supplement:
Movie 1. Control MO-injected Xenopus embryos gastrulate normally. Vegetal view of a typical embryo from the same clutch injected with 10 µM control MO that varies at five positions from the MO targeting MHC-B. Time-lapse imaging beginning at control stage 11 on MetaMorph at 6× magnification and with a 2-minute framing interval.
Movie 2. MHC-B morphants fail to gastrulate. Vegetal view of a typical embryo injected with 10 µM MHC-B MO. Time-lapse imaging beginning at control stage 11 on MetaMorph at 6× magnification and with a 2-minute framing interval.
Movie 3. Confocal imaging of aberrant motility of mosaic MHC-B-depleted cells. Axial explant with endodermal epithelium removed imaged from the mesodermal side with 5 µM MHC-B MO- and ruby-dextran-injected cells, in a background of cells expressing membrane-targeted GFP. Time-lapse confocal imaging of notochord beginning at control stage 13 on MetaMorph at 60× magnification and with a 15-second framing interval.
Movie 4. Mosaic MHC-B-depleted notochordal cells exhibit aberrant shape and motility, and eventually leave the notochord. Axial explant with endodermal epithelium removed and imaged from the mesodermal side with 5 µM MHC-B MO- and ruby-dextran-injected cells. Time-lapse fluorescent imaging of notochord beginning at control stage 13 on MetaMorph at 30× magnification and with a 1-minute framing interval.
Movie 5. Dynamic cortical actin visualized with a C-terminal moesin-GFP probe in notochordal cells. Confocal imaging of cortical actin reveals a basket-like appearance with multiple foci per cell at which actin gathers. Time-lapse confocal imaging of notochord at control stage 13/14 on MetaMorph at 120× magnification and with a 15-second framing interval.
Movie 6. MHC-B-depleted notochordal cell exhibits abnormal cortical actin morphology and dynamics. Confocal imaging of cortical actin in a mosaically MHC-B-depleted notochordal cell reveals sparser, thicker actin cables in cells. Time-lapse confocal imaging of notochord at control stage 13/14 on MetaMorph at 150× magnification and at a 15-second framing interval.
Movie 7. MHC-B-depleted notochordal cell exhibiting transition between normal and perturbed cortical actin dynamics. Confocal imaging of cortical actin in a mosaically MHC-B-depleted notochordal cell reveals sparser, thicker actin cables. Time-lapse confocal imaging of notochord at control stage 13/14 on MetaMorph at 150× magnification and at a 15-second framing interval.
Movie 8. Notochordal cells with a mild depletion of MHC-B exhibit profuse filopodia. Confocal imaging of mosaic notochordal cells injected with 1 µM MHC-B MO exhibit abnormally regulated cell motility. Time-lapse confocal imaging of notochord at control stage 13/14 on MetaMorph at 150× magnification and at a 15-second framing interval.
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