spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Myosin IIB MO-mediated failure of gastrulation is dose dependent. (A-D) Still images from simultaneous time-lapse videorecordings showing vegetal views of control (A) and morphant Xenopus embryos injected with 2.5 µM (B), 5 µM (C) and 10 µM (D) MHC-B MO. Embryos are oriented with their dorsal side up. At stage 10 (t=0), bottle cells form in the dorsal lip of the blastopore of all control and morphant embryos. By control stage 12 (t=2.5), ventral bottle cells have formed in all control and morphant embryos, but blastopore closure is delayed in a MHC-B MO dose-dependent manner. The site of blastopore closure in 2.5 µM (B, t=7.5) morphant embryos is not located as ventrally as in control embryos (A, t=7.5). Asterisks indicate the center of the yolk plug at t=0 and the point of blastopore closure at t=7.5. (E-H) RNA in situ hybridizations of stage-13 embryos for brachyury expression reveals the extent of notochordal morphogenesis in a control embryo (E), and reduced notochordal morphogenesis in a 5 µM morphant (F). The 5 µM morphant embryos exhibit some variability in notochordal extension (G), but 10 µM morphant embryos essentially lack notochord extension (H).





Right arrow Return to article