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Fig. 6. Cell-cell and cell-matrix adhesion are affected in morphant cells.
(A) Morphant dorsal axial cells at stage 13 exhibit a dramatic
dose-dependent reduction in adhesion to recombinant extracellular domain of
C-cadherin (C-Cad). (B) However, cell-surface levels of C-Cad are
unchanged in morphant cells, as similar levels of control and morphant cell
C-Cad is available on the cell surface for trypsin digestion. (C)
Morphant cells also show a dramatic reduction in adhesion to fibronectin (FN).
(D) Cell-surface levels of integrin 
5 are not affected by myosin
IIB depletion. (E) Failure of blastopore closure due to MHC-B depletion
is not rescued by exogenous expression of C-Cad, consistent with the
hypothesis that C-Cad levels are not limiting in morphant embryos. (F)
Adhesion to FN in morphant cells is rescued by co-expression of exogenous
human MHC-B.
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