spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. lvnumb is transcribed beginning at hatched blastula and remains expressed until the end of embryogenesis in the endomesoderm. The protein is localized to the presumptive NSM. (A) Northern blot of LvNumb expression during early development (5 µg/lane). Ethidium bromide staining of the 18s rRNA bands used as a loading control. A single transcript accumulates beginning at hatched blastula stage and is expressed throughout the rest of development. 16, 16-cell stage; HB, hatched blastula; MB, mesenchyme blastula; EG, early gastrula; LG, late gastrula; PL, pluteus larvae. (B) lvnumb expression is upregulated at the vegetal plate beginning at hatched blastula stage. (a,b) lvnumb transcripts are not localized in the egg or 60-cell stage embryos. (c) Beginning at hatched blastula, lvnumb is upregulated in the vegetal plate (region of thickened epithelia). (d) lvnumb maintains high expression in the vegetal plate region of mesenchyme blastula embryos. (e) lvnumb is expressed in involuting endoderm, but not delaminating secondary mesenchyme cells in early gastrula embryos (arrowheads indicate SMCs). (f) By late gastrula, lvnumb is expressed primarily in the hindgut and the foregut (arrowheads), but is largely excluded from the midgut. (C) (a-c) In the blastula, LvNumb protein is apically localized to the presumptive NSM that has been cleared of LvNotch. LvNotch (a) and LvNumb (b) staining and merge (c) showing overlap at the edge of the NSM field (arrowhead). (d-f) Apical LvNumb expression expands with the presumptive NSM territory in early gastrula embryos. LvNotch (d) and LvNumb (e) staining and merge (f) showing overlap at the edge of the NSM field (arrowheads). (g-i) In gastrula embryos, the apical expression of LvNumb has expanded into the endoderm, significantly overlapping with dorsal LvNotch expression. (g) LvNotch staining. (h) LvNumb staining. (i) Merged image of LvNotch and LvNumb protein expression. Arrowhead indicates where LvNumb and LvNotch overlap.





Right arrow Return to article