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Figure 1


Fig. 1. c-myc null and Sox2-Cre-mediated c-myc deletion result in anemia, vascular defects and lethality. (A-H) Anti-CD31-stained whole-mount embryos (A,B,E,F) and yolk sacs (C,D,G,H) dissected at 8.75 dpc (A-D) or 10.25 dpc (E-H). (I,J) Cross-sections (5 µm) of anti-CD31-stained whole-mount embryos. (K-N) Live embryos dissected at 11.5 dpc. (O-T) Anti-CD31-stained whole-mount yolk sacs (O,P) and heads (Q-T) at 11.5 dpc. Note the less developed vessels with reduced caliber (yolk sac) and number of branches (head) in the mutant animal. Da, dorsal aorta; ISV, intersomitic vessel; pp, primitive plexus; vv, vitelline vessels; CA, carotid artery; HV, primary head veins; CV, cardinal vein; nt, neural tube; Ph, pharynx. Scale bars: 200 µm.





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