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Figure 7


Fig. 7. Expression of proangiogenic factors in Vav-iCre;c-mycflox/- and Tie2-Cre;c-mycflox/- mutant embryos. (A) mRNA levels of proangiogenic factors in Vav-iCre;c-mycflox/- at 11.25 dpc. Real-time PCR was performed using total RNA from five mutants and 15 control embryos (Vav-iCre;c-mycflox/+, c-myc+/- or Vav-iCre;c-myc+/-). The statistical significance of the differences in means between controls (relative expression=1) and mutants are listed by their P values. (B) Increase in VEGF protein levels by ELISA in control and Tie2-Cre;c-mycflox/- embryos (n=5). VEGF levels in the Tie2-Cre mutant embryos were significantly elevated (*P<0.01).





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