|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Replication, sprouting and migration in Nrp1-/- yolk
sacs. (A) Endothelial replication was quantitated using double
staining with phospho-histone 3 and Pecam1 at different stages of development.
No statistically significant difference was seen at any of the stages
examined. (B) The number of Pecam1-positive unconnected segments
extending into avascular spaces, which may represent sprouts or regressing
vessels, was assessed from images of the yolk sac at E9.5. No significant
differences were observed between the different groups. (C-G)
Endothelial cell migration was assessed by focal injection of cell tracker
CM-DiI into a capillary region of the yolk sac followed by quantification of
the maximum migration distance per embryo (C). The initial injection site can
be observed in the white light images (red arrows, D,E). Endothelial cells did
not migrate very far away from the site of injection in wild-type embryos (F)
but were seen migrating longer distances along existing capillaries for
knockout embryos (arrowheads, G). *P<0.05, Student's
two tailed t-test. Scale bar: 1 mm.
|
