QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 6. Arterial venous gene expression in Nrp1^-/- embryos. (A-F) Serial sections were stained by in situ hybridization for Dll4 (A,B), Cx40 (C,D) and Efnb2 (E,F) in both wild-type (A,C,E) and Nrp1 knockout embryos (B,D,F) at 20 somites. Images show the common atrial chamber (CAC), the vitelline artery (VA) and the dorsal aortae (DA) at either the level of the heart (A-D) or at the caudal end of the embryo (E,F). (B) Red arrow indicates a missing dorsal aorta in the Nrp1^-/- embryo; the remaining dorsal aorta still expresses Dll4. (D) Cx40 expression is absent in Nrp1^-/- embryos. (F) Normal expression of Efbn2 is present in dorsal aorta and somites of Nrp1^-/- embryos, but lacking in the vitelline artery. (G) Levels of gene expression were measured from isolated endothelial cells, either pooled wild-type/heterozygous (denoted wild-type) or knockout, both with and without blood flow for 11 cardiovascular genes: four pan-endothelial genes, four arterial genes and three venous genes. Gene expression was normalized to three housekeeping genes: -tubulin, β-actin and Gapdh. Expression of Cx40 and Efbn2 in Nrp1^-/- is downregulated compared with wild type, regardless of flow environment. ^*P<0.05, ^**P<0.01, ^***P<0.005; Student's two-tailed t-test. Scale bars: 100 µm.

Return to article