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Fig. 3. Generation of branched ureteric systems from the `wrong end' of the
ureters of more mature kidneys. (A) A cut ureteric stalk of an
E12.5 kidney capped with E11.5 metanephric mesenchyme ramified through the
mesenchyme to generate a branched collecting duct system (circled). (B)
Branching morphogenesis of an E11.5 de-tipped stalk transferred to matrigel
with GDNF, FGF1 and pleiotrophin, incubated for 144 hours and stained with
FITC-phalloidin to reveal its anatomy. It is interesting to note that the
phalloidin stain is particularly strong in the apical regions of cells at the
branch tips, as described for normal ureteric buds developing in whole kidneys
(Michael et al., 2005).
(C,D) Tips formed from the `wrong end' of the ureter, as in A,
induce the formation of nephrons in the surrounding mesenchyme. These are not
detectable in the ureteric bud-specific anti-calbindin stain (C) but are
visible in the anti-laminin stain (D, arrow).
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