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Fig. 7. Upregulation of proneural genes in the dorsal telencephalic midline of
Hes1;Hes3;Hes5 cKO mice. (A-D)
Neurogenesis (Tuj1+) was enhanced in the dorsal telencephalic
midline (brackets) of Hes1;Hes3;Hes5 cKO mice at
E10.5 and E11.5 (B,D, asterisks), compared with the control (A,C).
(E,F) Double immunostaining for Ngn2 and Hes1 in wild-type
embryos. Many cells co-expressed Ngn2 and Hes1 at E10.5 (arrowheads), but the
expression was mostly segregated at E11.5 (arrows). (G-S) In the dorsal
telencephalic midline region (Lmx1a+) of control mice,
Ngn1 and Ngn2 expression occurred at low levels in subsets
of cells at E10.5 (G-I) and was down-regulated at E11.5 (J,K,Q,Q'). By
contrast, Ngn1 and Ngn2 expression was highly upregulated in
Hes1;Hes3;Hes5 cKO mice at both E10.5 and E11.5
(L-P, asterisks; R,R',S). *P<0.01;
**P<0.001, t-test. Scale bars: 50 µm in
A-D,E1,F1,G-I,L-N; 100 µm in J,K,O,P; 20 µm in Q-R'.
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