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Figure 1


Fig. 1. A BAC enhancer scan across the mouse Tbx4 region. (A) Schematic of genomic region encompassing Tbx4. Arrows denote the direction of transcription of Tbx4 and flanking genes. Gray bars show BAC locations with the inserted IRES-βGeo cassette in blue. Mapped cis-regulatory intervals are shaded: yellow, hindlimb interval I; green, lung interval; pink, hindlimb interval II. (B,C) Whole-mount in situ hybridization for Tbx4 mRNA in E12.5 mouse embryos. (D-I) Whole-mount lacZ staining of transgenic embryos carrying RP24-376P4, RP24-84E15 or RP23-136J3. Dotted lines denote boundaries of limbs, umbilical cord (uc) and genital tubercle (gt). lu, lung.





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