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Fig. 2. Comparative genomics reveals locations of candidate cis-regulatory
elements. (A) Sequence comparison of mouse with human, opossum,
chicken and zebrafish Tbx4. Colored bars (top) denote location of
cis-regulatory intervals; blue boxes indicate position of Tbx2 and
Tbx4 exons. All regions with >70% identity over a 100 bp window
are colored: blue, exons; red, conserved intergenic sequence; pink, conserved
intronic sequences). Block-shaded portions of plots indicate positions of
confirmed enhancers (see B): yellow, hindlimb enhancer A (HLEA); green, lung
enhancer; pink, hindlimb enhancer B (HLEB); gray, umbilical cord. (B)
Genomic regions assayed for enhancer activity. Black bars represent fragments
that did not drive consistent expression patterns at E12.5. (C-G)
Whole-mount lacZ staining of E12.5 transgenic mouse embryos
illustrating expression patterns seen with the different constructs. tr,
trachea; lu, lung; uc, umbilical cord.
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