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Figure 4


Fig. 4. Developmental time course of HLEA and HLEB activity compared with the endogenous expression of Tbx4. (A-F) Whole-mount lacZ staining of mouse embryos from stable transgenic lines carrying HLEA transgene pDBM7 (A-C) or HLEB transgene pDBM5 (D-F) show that these enhancers are active in the early hindlimb field (A,D) and developing hindlimb bud (B,C,E,F). (G-I) Tbx4 mRNA as detected by whole-mount in situ hybridization shows similar patterns of expression. Embryonic stages as indicated.





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