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Figure 5


Fig. 5. HLEA and HLEB are both required for robust hindlimb expression of Tbx4. (A) Schematic of mouse Tbx4 genomic region. BAC transgene RP24-84E15 and derivatives are represented by gray bars. Yellow ovals, HLEA; green circles, lung enhancer; gray circles, umbilical cord element; pink ovals, HLEB; blue box, IRES-βGeo. (B-I) Lateral (B,D,F,H) and ventral-posterior (C,E,G,I) views of whole-mount lacZ staining of E12.5 transgenic mouse embryos carrying BAC transgenes RP24-84E15 (B,C), {Delta}HLEA (D,E), {Delta}HLEB (F,G) or {Delta}HLEA{Delta}HLEB (H,I).





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