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Figure 5


Fig. 5. Effects of the FGF signaling pathway on the regulation of Mesp2 expression. (A-C) The spatial relationship between Mesp2 (using mRNA probe) and Dusp4 was examined by double in situ hybridization. The posterior border of the Mesp2 expression domain (round bracket in B) coincides with the anterior limit of the Dusp4 expression domain (border indicated by arrow in A,B). The stage of this embryo was assigned as phase I. A higher magnification image of B is shown in C. (D,E) Dusp4 expression revealed by whole-mount in situ hybridization in wild-type (D, n=4) and Mesp2-null (P2L/P2L; E, n=2) embryos. (F) An analysis of PSM-specific Fgfr1 knockout (cKO) mice. Whole-mount in situ hybridization revealed a posterior shift of the Mesp2 expression domain in the Fgfr1-cKO embryo (n=8). (G) Caudal portions of E10.5 embryos were treated with FGF inhibitor (SU5402) or DMSO (control) for 6 hours. A posterior shift of the Mesp2 expression domain was observed in the embryos treated with SU5402 (5 out of 6 embryos). (H) Caudal portions of E10 embryos were bisected and the left halves were treated with DMSO (control), while the right halves were treated with SU5402. A posterior shift of the Mesp2 expression domain was observed in 10 out of 13 SU5402-treated embryos tested. (I-L) Double immunostaining of sections was employed to examine Tbx6 and Mesp2 (I,J) or Mesp2 and NICD (K,L) expression in wild-type control (I,K) and Fgfr1-cKO (J,L) embryos.





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