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Fig. 1. Immunohistochemical analysis of KLF5 in the developing mouse lung.
(A) At E12.5, during the early pseudoglandular stage of development,
KLF5 was detected in nuclei of epithelial cells in the proximal bronchial
tubules (arrow). Staining was generally more intense along medial aspects of
the tubules. ES, esophagus. Arrowhead indicates peripheral epithelial cells.
(B) At E15.5, KLF5 was detected at highest levels in bronchiolar
tubules (arrow); however, staining of subsets of cells lining the peripheral
lung buds (arrowhead) was also noted. (C) At E18.5, KLF5 was present in
both peripheral (arrowhead) and proximal (arrow) airways. (D) In mature
lung, KLF5 was detected in nuclei of subsets of epithelial cells in both
conducting airways (arrow) and alveolar regions (arrowhead). Insets are higher
magnifications of the corresponding figures. (E-G) Dual immunolabeling
for KLF5 (green nuclei) and (E) proSPC (red cytoplasm), (F) CCSP (red
cytoplasm), and (G) FOXJ1 (red nuclei) was performed on E18.5 lung sections.
KLF5 staining was observed in proSPC-positive (large arrow) and -negative
(small arrow) cells (E), in CCSP-positive cells (large arrow, F), and in
FOXJ1-negative cells (small arrow, G). Yellow signal is due to the
autofluoresence of red blood cells, detectable in both channels. Scale bars:
100 µm.
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