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Fig. 3. Deletion of Klf5 disrupts lung morphogenesis. (A-H)
Lung sections were prepared from the fetus at E15.5 (A,B) and E18.5 (C-H), and
were stained with a guinea pig anti-mouse KLF5 polyclonal antibody (A-F) or
pancytokeratin (G,H), which outlined the epithelial cells. Nuclear staining of
KLF5 was observed in epithelial cells lining the conducting and peripheral
airways in control (A,C) and Klf5
/+ (E) mice.
Staining was absent or decreased in Klf5/
mice (B,D,F). Morphological changes in the lungs of
Klf5/ mice were not observed at E15.5 (A
versus B). At E18.5, lung morphology was markedly perturbed in
Klf5/ mice (D,F,H), note the thickened walls
of the mutant saccules, compared with Klf5+/+ (C,G) and
Klf5/+ mice (E). Squamous epithelial cells (arrow)
were rarely observed in the lungs of Klf5/
mice (H), compared with controls (G). Scale bars: 50 µm.
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