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Fig. 1. The DSL ligand lag-2 is expressed in the three pairs of IL2
neurons during the dauer stage. (A) A daf-7(e1372) dauer
larva expressing the lag-2::GFP transgene and the corresponding DIC
image overlaid with the GFP expression to show the position of the cells
expressing the transgene. White arrowheads indicate the described expression
of lag-2::GFP in the distal tip cells (DTC). Scale bar: 25 µm.
(B) The head region of dauers expressing lag-2::GFP (qIs56)
induced by either pheromone, or in various Daf-c mutants as indicated
in the panels. (C) 3D reconstruction of a confocal stack of images of
the IL2 neurons in dauer. White lines outline the pharynx of the dauer animal.
Scale bars: 10 µm. (D) Diagram of the IL2 neurons indicating their
position and their characteristic morphology (adapted with permission from
wormatlas.org).
(E) Merge of dauer expressing lag-2::GFP (green) in the IL2
neurons that were stained with the lipophilic dye DiI (red). As
lag-2::GFP is expressed in the entire cell, whereas DiI only stains
the membrane, colocalisation (yellow) is only observed at the membrane, giving
a halo-like appearance. In all images, arrowheads indicate the IL2
neurons.
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