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Fig. 6. Sf1/Cre^low-driven loss of β-catenin permits adrenal survival owing to incomplete inactivation of β-catenin. (A) Photograph of the urogenital region of newborn (P0) WT, Sf1/Cre^low- and Sf1/Cre^high-β-catenin KO mice. H&E staining of sagittal sections in the region of the adrenal gland from P0 wild-type mice or from mice with β-catenin KO mediated by the indicated Sf1/Cre transgenes. (B) LacZ stained sections of Wnt-Gal and Wnt-Gal/β-catenin KO^low, as described in the Materials and methods. Scale bars: 200 µm. (C) Quantitation of active canonical Wnt signaling by comparing the area of lacZ staining versus the total adrenal area (P<0.05 versus Cre-negative littermates). Quantitative-PCR analysis of Axin2 expression in adrenal glands from wild-type and Sf1/Cre^low-β-catenin KO mice (P<0.05 versus Cre-negative littermates). Ad, adrenal gland; K, kidney; l, liver. (D) Demonstration of recombination of the β-catenin gene in DNA samples from wild-type mice and those with β-catenin inactivation mediated by the Sf1/Cre^low transgene.

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