|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 10. kit mutant reveals critical period for ErbB activity during
neural crest migration. Embryos were reared to juvenile stages after
treatment with AG1478 as embryos. (A) Normal kit mutant
pigment pattern after AG1478 treatment between 26 and 30 hpf (stripe break
score=0; for details see Materials and methods). kit mutant adults
exhibit about half as many stripe melanophores as wild-type fish and
completely lack melanophores over the dorsum and on scales
(Johnson et al., 1995).
(B,C) Treatment with AG1478 between 14 and 18 hpf results in
pattern defects that are moderate (B, stripe break score=3) to severe (C,
stripe break score=4). (D) Distinct melanophore-free patches (arrows)
in kit mutant treated between 8 and 11 hpf then again between 26 and
30 hpf. (E,F) Quantitation of stripe break defects in
kit mutants treated with AG1478 in two separate experiments. Shown
are median stripe break scores (red bars) and interquartile ranges (50% of
scores, black vertical bars). Numbers above each treatment stage are sample
sizes of adult fish analyzed. con, DMSO-treated controls. (E) Initial
experiment reveals peak sensitivity between 16 and 22 hpf, with lesser defects
extending between 8 and 36 hpf (test of differences in median locations across
all treatments, Wilcoxon test 
2 approximation=38.6, d.f.=11,
P<0.0001). (F) Second experiment confirms peak sensitivity between
14 and 22 hpf (2=115.6, d.f.=14, P<0.0001).
More severe defects are generated with repeated treatments (right).
|
