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Fig. 6. Autonomous and non-autonomous roles for erbb3b in pigment
pattern metamorphosis. (A) Wild-type 
picasso
chimeras frequently developed wild-type melanophores in stripes at high
density anteriorly (arrows, left) but at lower density in the mid-trunk (small
arrows, right; 75% of chimeras developed donor melanophores; chimeras with
donor cells and total reared: n=24, 64, respectively). A wild-type
midbody lateral line is misrouted as well. (B) Melanophores at high
density anteriorly that are either donor-derived (EGFP+) or
host-derived (EGFP-). (C) Melanophores in the mid-trunk are
more spread, which is typical at low density. In reciprocal picasso
wild-type chimeras, we did not observe donor metamorphic melanophores
(n=7, 50). (D) Wild-type nacre chimeras
developed patches of donor-derived metamorphic melanophores that populated
stripes (arrow) and scales (84% of chimeras developed metamorphic
melanophores; n=75, 155). Persisting embryonic/early larval
melanophores (arrowheads) are identifiable by location, large size and browner
color (Quigley et al., 2004).
(E) picasso nacre chimeras developed
melanophores (arrowheads), but did not develop metamorphic melanophores [79%
of chimeras developed embryonic/early larval melanophores or fin melanophores
(not shown); n=58, 195]. Donor cells in all chimera combinations
contributed at similar frequencies to other derivatives, including muscle,
epidermis, eye and neurons of the lateral line. Scale bars: in A, 500 µm;
in B, 200 µm for B,C; in D, 1 mm for D,E.
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