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Fig. 7. Embryonic requirement for ErbB signaling in pigment pattern
metamorphosis. (A) Control wild-type zebrafish treated from
embryonic through juvenile stages with DMSO alone. (B) Wild-type
treated with AG1478 during embryonic stages (70% epiboly - 4 dpf) showed adult
pigment patterns resembling severely affected picasso mutants
(arrow). (C,D) Individuals treated with AG1478 during the
pre-metamorphic, early larval period (5-14 dpf, C) or throughout metamorphosis
(15-28 dpf, D) exhibited pigment patterns indistinguishable from controls.
(E) Metamorphic melanophore densities in the mid-trunk (mean±95%
confidence intervals) showing similarities between wild-type (untreated),
control (con, DMSO-treated) and fish treated with AG1478 during
pre-metamorphosis (pre) and metamorphosis (met), as well as similar defects
between picasso mutants (pcs) and fish treated with AG1478
as embryos (emb). Letters above bars indicate means that are not significantly
different (P>0.05). Numbers within bars are samples sizes.
(F,G) D. albolineatus normally develop a more uniform
melanophore pattern of metamorphic melanophores (arrowheads in F) compared
with D. rerio, and exhibited a severe melanophore deficiency when
embryos were treated with AG1478 (G). (H,I) In D.
nigrofasciatus, adult stripes largely comprise persisting embryonic/early
larval melanophores with occasional gaps (arrow in H), and embryonic treatment
with AG1478 had relatively subtle effects (arrows in I). (J,K)
Embryonic morpholino knockdown of erbb3b resulted in adult
melanophore deficiencies (K) compared with controls (J), providing independent
evidence for an early erbb3b-dependence of adult pigment pattern
formation. Scale bar: in I, 0.5 mm for A-D,F-I,J,K.
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