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Fig. 1. erbb3b and erbb2 mutants lack DRG and sympathetic
neurons. (A-C) Elavl antibody labeling reveals DRG (arrowheads) and
sympathetic neurons (asterisks). DRGs are segmentally arranged, whereas at
this stage, sympathetics are not segmental. DRG and sympathetic neurons are
present in wild-type embryos (A) but are absent from erbb3b (B) and
erbb2 (C) mutants at 7 dpf. (D-F) Elavl-positive enteric
neurons appear normal in erbb3b (E) and erbb2 (F) mutants
compared with wild-type embryos (D). (G-I) TH antibody labeling reveals
sympathetic neurons. Whole-mount labeling of 8 dpf larvae showing that
sympathetic neurons (asterisks) are present in wild type (G) but absent from
erbb3b (H) and erbb2 (I) mutants. (J-L) Whole-mount
labeling of 31 hpf embryos with a neurog1 riboprobe reveals nascent
DRG neurons. Nascent DRG neurons (arrowheads) are segmental in wild-type
embryos (J) but absent from erbb3b (K) and erbb2 (L)
mutants. (M-O) A neurod riboprobe reveals DRG neurons at 36
hpf. DRG neurons (arrowheads) are segmental in wild-type embryos (M) but
absent from erbb3b (N) and erbb2 (O) mutants. Lateral views;
anterior towards the left. Scale bar: 20 µm.
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