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Fig. 5. nrg1 alone is not required for DRG formation. (A)
RT-PCR showing expression of each nrg1 isoform at various
developmental stages and in the adult brain. (B,D,F) Expression patterns of
each isoform at 24 hpf shown in whole-mount. (C,E,G) Cross section of
expression pattern of each isoform respectively. (B,C) Type I is
expressed weakly in somites. (D,E) Type II and
(F,G) type III are expressed strongly in ventral spinal cord
neurons. (E,F) Elavl labeling at 2 dpf. (H,I) Control
MO-injected embryos (H) had segmental DRG neurons. nrg1 MO-injected
embryos (I) had only a slight decreased in DRG neuron number; arrowhead
indicates absent DRG, asterisk shows mislocalized DRG. Scale bar: 40
µm.