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Figure 1


Fig. 1. PADI6 is required for cytoplasmic lattice formation in oocytes and two-cell embryos. (A) Padi6+/+ primordial follicle oocyte. N, nucleus; C, cytoplasm. (B) Padi6-/- primordial follicle oocyte. (C) CPLs are first observed in Padi6+/+ growing oocytes. Rb, ribosome; IS, intermediate structure; CPL, cytoplasmic lattice. (D) CPLs do not form in Padi6-/- growing oocytes. (E) CPLs are also observed in Padi6+/+ fully grown germinal vesicle stage oocytes. (F) CPLs are not observed in Padi6-/- fully grown germinal vesicle stage oocytes. (G) Arrows highlight CPLs in Padi6+/+ two-cell embryos. (H) CPLs are not observed in Padi6-/- two-cell embryos. Scale bar: 200 nm. These experiments were repeated twice.





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