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Fig. 2. Ribosomal components display increased solubility in
Padi6-/- oocytes. (A) qRT-PCR analysis using
18S rRNA primers and western blot analysis using anti-S6 antibodies indicates
that levels of ribosomal components are similar between
Padi6+/+ and Padi6-/- oocytes.
(B) In contrast with Padi6+/+ oocytes, the majority
of ribosomal protein S6 partitions in the supernatant of ruptured
Padi6-/- oocytes. Oocytes were ruptured in hypotonic
buffer, serially centrifuged at 650 and 9000 g for 5 minutes,
and the partitioning of ribosomal components into the supernatant (Sup) and
pellet fractions was evaluated by immuno-slot-blot analysis using anti-S6
antibodies. (C) Transmission electron microscopic analysis indicates
that although putative CPLs (arrow) are observed in the
Padi6+/+ 9000 g oocyte pellet, (D)
lattices are not observed in the Padi6-/- oocyte pellet.
Arrowheads highlight ribosome-like particles associated with CPLs. Scale
bar:100 nm. Values for the qRT-PCR are represented as the mean±s.e.m.
from three independent experiments (P=0.407). The immunoblotting
experiments were repeated three times and the electron microscopy experiments
were repeated twice.
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