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Figure 3


Fig. 3. PADI6 and ribosomal protein S6 colocalize in Triton X-100 extracted oocytes and loss of PADI6 alters S6 localization but not de novo protein synthesis. (A) PADI6 (P6) colocalizes with ribosomal protein S6 in Triton X-100 extracted GV stage Padi6+/+ oocytes and S6 localization is altered in Padi6-/- oocytes. PADI6 and S6 co-localization in Padi6+/+ oocytes is highlighted in merged image (M). The degree of co-localization (oval) is shown in the scatter plot. S6 localization to the oocyte cortex (or lack thereof in the Padi6-/- oocytes) is highlighted by the arrowheads. (B) Phalloidin staining of Triton X-100 extracted GV oocytes reveals that the cortical actin network is not disrupted in Padi6-/- oocytes. (C) Wide-field immunofluorescence analysis shows that DNMT1 displays similar cortical localization in Padi6 +/+ and Padi6-/- GV stage oocytes. (D) Fluorographic analysis of [35S]methionine labeled proteins from Padi6+/+ and Padi6-/- GV stage oocytes. Arrow indicates a protein that, based on its molecular weight (~72 kDa) and absence from Padi6-/-oocytes, is probably PADI6. These experiments were repeated three times.





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