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Figure 4


Fig. 4. Loss of PADI6 alters the levels and localization of ribosomal protein S6 and affects protein synthesis in two-cell embryos. (A) PADI6 (P6) colocalizes with S6 at the non-apposed cortical regions of Padi6+/+two-cell embryo blastomeres, whereas S6 is absent from the embryonic cortex in Padi6-/- two-cell embryos (arrows and B). (C) Phalloidin staining reveals that the cortical actin network is not disrupted in Padi6-/- two cell embryos. (D) Western blot analysis of protein extracts indicates that S6 levels are reduced in Padi6-/- two-cell embryos. (E) Fluorographic analysis of [35S]methionine labeled proteins indicates that specific mRNAs are translated at different efficiencies in Padi6-/- two-cell embryos. Asterisk indicates putative spindlin protein. (F) Confocal immunofluorescence analysis confirms that spindlin expression is upregulated in Padi6-/- two-cell embryos. These experiments were repeated three times.





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