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Figure 5


Fig. 5. Embryonic genome activation is defective in Padi6-/- two-cell embryos. (A) Levels of RNA polymerase II (RNA Pol II), phosphorylated RNA Pol II (P-Ser2-CTD) and acetylated histone H4 (H4K5 acetyl) are significantly reduced in Padi6-/- two-cell embryos. Representative nuclei shown were chosen based on their average signal intensity matching the respective mean of average intensities for Padi6+/+ and Padi6-/- embryo nuclei. Values represent the mean signal intensity±s.e.m. (B) BrUTP incorporation into the nucleus of Padi6-/- two-cell embryos is significantly reduced. Representative nuclei are shown above bars in histogram. Values represent the mean BrUTP nuclear incorporation±s.e.m. from three independent experiments (P<0.05). (C) Fluorographic analysis of [35S]methionine labeled and Triton X-100 extracted two-cell embryos indicates that synthesis of transcription requiring complex (TRC) proteins is reduced in Padi6-/- embryos. These experiments were repeated three times.





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