spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Targeted presynaptic dFMRP rescues all dfmr1-null NMJ structure defects. (A) Representative images of wandering third instar Drosophila larval NMJs; genotypes and treatments as shown. NMJs co-labeled for HRP (presynaptic marker) and DLG (postsynaptic marker), with three examples of each condition shown. (B-D) Quantification of NMJ synaptic branch number and area, defined for presynaptic area (HRP domain) and postsynaptic area (DLG domain). dfmr1-null terminals display overgrowth and over-elaboration. GS+E phenocopies dfmr1, and complete rescue occurs with RU486 constitutively driving presynaptic dFMRP expression. (E) Representative images of mini/satellite boutons; genotypes and treatments as shown. High-magnification images of boxed regions in A showing mini-boutons (arrowheads) in both dfmr1-null and GS+E genotypes and their absence following RU486 induction. (F,G) Quantification of normal (>2 µm diameter) and mini-bouton (≤2 µm diameter, attached to a normal bouton) number per NMJ in genotypes and treatments shown. n=12 animals for all conditions. Bars indicate mean±s.e.m. Dashed red lines highlight control level quantifications. **P<0.01, ***P<0.001.





Right arrow Return to article