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Fig. 1. Induction of Cx43 expression in uterine stromal cells during
implantation. (A) Mice were subjected to delayed implantation as
described in the Materials and methods. Uteri were collected 1 hour (b) and 24
hours (c,d) after E treatment and subjected to immunohistochemistry using
anti-Cx43 antibody. (d) Higher magnification view of the 24-hour sample. The
0-hour sample (a) represents pregnant uterus obtained from a mouse treated
with P alone for three days. Results are from two independent experiments.
(B) Uteri were collected in the morning of day 1 (a), day 4 (b), day 5
(c,d) and day 7 (e,f) of pregnancy. Higher magnifications of Cx43 expression
in stromal cells surrounding the implanted embryo on day 5 and day 7 are shown
in d and f, respectively. Results are from two independent experiments.
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