QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 3. Impaired embryo development and lack of angiogenesis in uteri of Cx43 conditional knockout mice on day 8 of gestation. (A) Hematoxylin and Eosin staining of uterine sections obtained from Cx43^fl/fl (a,b) and Cx43^d/d (c,d) mice on days 7 and 8 of pregnancy. Note that implanted embryos (E) develop poorly in Cx43^d/d mice compared with those in Cx43^fl/fl mice. Histological analysis by Hematoxylin and Eosin staining of perfused uterine sections obtained from pregnant Cx43^fl/fl (e) and Cx43^d/d (f) mice on day 8 of pregnancy. E, implanted embryo; M, the mesometrial region. Note the extensive vasculature in the uteri of Cx43^fl/fl mice and the lack of it in the uteri of Cx43^d/d mice. (B) Immunohistochemical staining (indicated by red deposits) for Pecam in pregnant uteri. Uterine sections of Cx43^fl/fl (a-c) and Cx43^d/d (d-f) mice on day 7 (a,b,d,e) and day 8 (c,f) of pregnancy are shown (n=3). (C) Uterine sections collected from mice on day 8 of pregnancy were subjected to immunoflouroscence employing anti-connexin 43 (a) and anti-Pecam1 (b) antibodies. c represents a merge of both Cx43 and Pecam staining indicating the expression of Cx43 in the stromal cells but not in the endothelial cells (n=2). (D) Uterine sections from Cx43^fl/fl (a) and Cx43^d/d (b) mice on day 8 of gestation were subjected to immunohistochemistry using an antibody against Ki67, a marker of cell proliferation (n=2).

Return to article