spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. FGF is required for rosette assembly, a prerequisite for primordium migration. Primordia of cxcr4b mutant embryos treated with DMSO (A) or SU5402 (B), showing the complete disappearance of rosettes after blocking the FGF pathway for 6 hours. (C,D) Time-lapse analysis and corresponding kymograph showing the progressive melting of rosettes after treatment with 80 µM SU5402. The migration speed is normal (70 µm/hour) and remains constant during this phase. Scale bar: 50 µm. (E,F) Time-lapse analysis and corresponding kymograph of a washout experiment showing that five rosettes (red arrows in E,F) simultaneously reassemble before migration resumes. Three phases can be distinguished on the kymograph: (1) uncoordinated migration, (2) rosette reassembly, and (3) migration recovery. (G,H) Expression of Fgf ligands in cldnb:gfp embryos treated for 6 hours with SU5402. Lower panels are transmission images of NBT/BCIP stainings. fgf3 (G) and fgf10 (H) are strongly expressed throughout the primordium when Fgfr is blocked. Scale bars: 20 µm (unless stated otherwise).





Right arrow Return to article