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Fig. 6. Rosettes are radial clusters of apically constricted epithelial
cells. (A) Electron micrographs of an apical section of a rosette;
false-colouring highlights individual cells. (B) Close-up of the centre
of the rosette, corresponding to the framed area in A. (C-E)
Immunostaining using antibodies against GFP and ZO-1 (C), GFP and aPKC (D),
and GFP plus phalloidin staining (E). Tight junction protein ZO1 and aPKC are
only expressed at the center of each rosette; phalloidin-labeling reveals
enriched actin at centre of rosette. (F) Time-lapse analysis of
wild-type rosette formation reveals a coordinated constriction of the apical
surfaces of several cells. Arrows point to the vertices of these cells as they
coalesce. Scale bars: 20 µm (unless stated otherwise).
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