DEV021766 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Inhibition of compensatory apoptosis results in massive overproliferation in the compartment overexpressing crol. All discs are en-GAL4,UAS-GFP/UAS-Crol;UAS-p35/+. (A-C) PH3 staining (purple) of 96-hour discs. (D-F) BrdU labelling of 96-hour discs. The border of the PC is marked white line and a small patch of non-GFP cells are within the yellow line. (G-I) 120-hour discs stained with PH3 (purple), (J-L) BrdU labelling of 120-hour discs. The ZNC of the 120 hour discs with the ZNC marked with a white arrow. The border of the PC is marked white line and the patch of non-GFP cells are within the yellow line. (C,F,I,L) Merge including a DNA stain (blue) to show the AC. (M-O) Anti-En antibody stain (red) of 120-hour discs; (N) merged with GFP and (O) with GFP and DAPI (blue).

• Supplemental Figure S2 -

  Fig. S2. Inhibition of caspase dependent apoptosis via overexpression of p35 alone does not result in overproliferation. All discs are en-GAL4,UAS-GFP/+;UAS-p35/+ at 120 hours aed. (A) PH3 staining (purple); (B) PH3 merged with GFP; (C) Merge of PH3, GFP and DNA (blue). (D) BrdU labelling (red); (E) BrdU merged with GFP; (F) merge of BrdU, GFP and DNA (blue).

• Supplemental Figure S3 -

  Fig. S3. Crol does not affect wg transcription indirectly via the Hh or N pathways. (A-C) crol mutant clones in the Minute background marked by the absence of β-gal (green) stained with Ci antibody (red). (D) Control Ptc-GAL4, UAS-GFP/+ stained for En(spl)m7 (red), (E,F) Ptc-GAL4, UAS-GFP/UAS-crol;UAS-p35/+ discs with En(spl)m7, the boundary of Ptc expression is marked by a line in D and E.