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Figure 1


Fig. 1. Crol interacts with the Wg-inducible cell cycle inhibitor Hfp. (A) Hfp is upregulated by Wg and inhibits G1- to S-phase transition by downregulating dm and mitosis via Stg. (B-D) Halving the dose of crol enhances the hfp overexpression wing phenotype. Adult wings for en-GAL4/+ control (B), en-GAL4, UAS-hfp/+ (C) and en-GAL4, UAS-hfp/crol[k05205]; (D). A tracing of the posterior compartment from en-GAL4, UAS-hfp/+ has been superimposed on the en-GAL4, UAS-hfp/crol[k05205] wing. (E-G) Crol antibody (red) on wild-type third instar wing disc, merged with DNA (blue, F) and with Wg (G, green). (H-J) UAS-crol;UAS-p35 flip-out clones marked with GFP (H), with Crol antibody (red, I) and merged with DNA (blue, J).





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