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Figure 4


Fig. 4. Crol regulates G1-S and G2-M cell cycle genes. (A) Adult wings overexpressing UAS-crol with en-GAL4 and with the following cell cycle mutants: (B) dmPO, (C) cycEAR95, (D) stgAR2, (E) cycB2, (F) E2F1. A tracing of the PC from en-GAL4/+, UAS-crol/+ has been superimposed to show relative modification of compartment size. (G-I) β-gal (red) of 120-hour wing discs for PL35/+; en-GAL4,UAS-GFP/UAS-crol;UAS-p35/+. (J-L) β-gal (red) on 96-hour wing discs for stg-lacZ 6.4/+; Ptc-GAL4,UAS-GFP/UAS-crol; UAS-p35/+. (M) Control PCNA-GFP in the 120-hour wing disc with the PC marked by En (red). (N) en-GAL4/UAS-crol; UAS-p35/PCNA-GFP, merged with En antibody in red (O).





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