|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Positioning of postmitotic neurons was affected in CKO mutants.
(A-C) Birth-date analysis was carried out by CldU and IdU double
staining of E18.5 the dorsal pallium sections after a single pulse of CldU at
E13.5 and IdU at E15.5. (D-I) Quantification of CldU- and IdU-positive
cell index in each Bin. In wild-type embryos, E13.5-born neurons (CldU+, red)
mostly settled down in the deep cortical layers with a peak in Bin4
(**29.92±2.45%; n=3, in D) and E15.5-born neurons
(IdU+, green) distributed mainly to the superficial layer, Bin5
(#14.12±2.51%; n=3, in G). In Shh-CKO embryos,
E13.5-neurons positioned similarly to wild type though neurons in Bin4 were
significantly decreased (**11.24±1.89% in E compared with D;
n=3, P<0.01). E15.5 neurons in Shh-CKO were
positioned in the almost same pattern as in wild type (compare G with H). In
Smo-CKO embryos, E13.5 neurons were differently scattered throughout
the dorsal pallium (Bin1****, 4.06±0.669%;
Bin2***, 18.38±3.69%; Bin5*, 28.07±0.76%;
n=3, P<0.01 in F) compared with wild type
(Bin1****, 0.09±0.09%; Bin2***,
2.56±1.86%; Bin5*, 14.00±3.29%; n=3, in D).
E15.5 cells mostly remained in SVZ/VZ (Bin1 and 2 in I) and significantly
decreased in CP (Bin3##, 3.63±1.97%; n=3, P<0.05
and Bin5#, 1.51±0.38%; n=3, P<0.01 in I) compared
with wild type (Bin3##, 7.37±1.02% and Bin5#, 14.12±2.51%;
n=3, in G). Scale bar: 50 µm.
|
