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Fig. 1. CTCF depletion in oocytes and embryos. (A) Quantification of
nuclear CTCF immunofluorescence staining in growing oocytes. Ntg and Tg
indicate oocytes derived from 10-day-old Ntg and Tg littermates, respectively.
Lines 1, 12 and 21 are shown. *P<0.0001;
n=number of oocytes. (B) Immunofluorescence images of
CTCF-stained oocytes and embryos. One confocal section of an egg or embryo is
shown per panel, with red indicating CTCF-stained nuclei. Line 1 is shown.
Scale bar: 40 µm. (C) Schematic of CTCF depletion in oocytes and
embryos. Ntg females were mated to Tg males and 
50% of female progeny
inherited the transgene. All oocytes from Tg females were CTCF depleted.
CTCF-replete oocytes and embryos are depicted in brown, whereas CTCF-depleted
oocytes and embryos are depicted in white. Pink and blue circles represent
maternal and paternal pronuclei, respectively. GV, germinal vesicle; hr, hours
post-hCG.
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