|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. Early mitotic defects leading to apoptosis. (A) Embryo
development in culture. One-cell embryos were cultured to 72 hours post-hCG
and categorized according to cell number. Line 1 is shown. n=number
of embryos. (B) Autoradiogram and (C) quantification of TRC
upregulation. Two-cell embryos from Line 1 were radiolabeled at various
timepoints post-cleavage. At each timepoint, TRC expression in 3-5 embryos
derived from Tg mice were normalized to TRC expression in an equivalent number
of embryos derived from Ntg mice (relative expression=1). Early, Mid and Late
indicate embryos radiolabeled at 6, 12 and 21 hours post-cleavage;
*P<0.05; n=number of experiments. + indicates
Spindlin, an abundant maternal protein. (D) Embryo development in vivo.
Cleavage-stage embryos were flushed at 72 hours post-hCG and categorized
according to cell number. Lines 1, 12 and 21 are shown. n=number of
embryos. (E) Immunofluorescence images of DAPI-stained embryos in D.
One confocal section of an embryo is shown per panel, with green indicating
DAPI-stained nuclei. Line 1 is shown. Tg embryos have ectopic nuclei. Arrow,
large ectopic nucleus; arrowhead, small ectopic nucleus. Scale bar: 20 µm.
(F) Percentages of embryos in D with ectopic nuclei. Embryos were
categorized according to whether large (white bars) or small (gray bars)
ectopic nuclei were observed. Lines 1, 12 and 21 are shown.
*P<0.05; **P<0.0001;
n=number of embryos. (G) Apoptosis at 120 hours post-hCG.
One-cell embryos were cultured to 120 hours post-hCG and TUNEL stained. One
embryo is shown per panel, with green indicating DAPI-stained nuclei and red
indicating TUNEL staining. Line 1 is shown. Only 7% (2/28) of embryos derived
from Tg mice versus 94% (29/31) of embryos derived from Ntg littermates formed
a blastoceol cavity by 120 hours post-hCG. Scale bar: 40 µm.
|
