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Fig. 1. Pancreas-specific Rbpj and Notch1/2KO mice.
(A) Strategy for targeting the Rbpj locus to generate
Rbpj+/f mice. The loxP sequences (arrowheads),
exons (filled boxes), length of diagnostic restriction fragments and location
of a 3'-probe (bar) used for Southern blotting are shown. EcoRI
restriction enzyme sites (E) are indicated. (B) Southern blot analysis
of Cre-mediated deletion in the following organs of an
Ptf1a+/Cre(ex1);Rbpj+/- mouse: thymus (lane 1),
spleen (2), liver (3), pancreas (4), kidney (5), head (6), lung (7), salivary
gland (8), stomach (9), duodenum (10), and coecum (11). The positions and
sizes of the fragments derived from the wild-type (WT), deleted,
floxed and pseudogene alleles are indicated. (C) Newborn
RbpjKO mice show increasing signs of growth retardation and die 4-5
days postpartum. (D-I) Macroscopic (D-F) and microscopic (G-I) X-gal
staining analysis of intestinal tracts from newborn mice show
X-gal+ pancreata (blue) from RbpjKO;R26R, Notch1/2KO;R26R
and Rbpj+/-;R26R pups. Arrowhead in F indicates
weakly branched ducts in pancreatic rudiment. neor,
neomycin-resistance gene; HSV-tk, herpes simplex virus thymidine kinase gene;
d, duodenum; l, liver; p, pancreas; sp, spleen; st, stomach. Scale bar: 100
µm.
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