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Fig. 4. Appearance of acinar cells in late stage of embryonic development of
RbpjKO embryos. (A-F) Analysis of amylase expression at
E14.5 (A-C) and E18.5 (D-F) in mutant pancreata. (G-I) Immunostaining
of mutant pancreata with an antibody against the ductal marker CK19 (black,
arrows) at day 3 postpartum (P3). (I) CK19 and X-gal (blue) co-staining of
RbpjKO pancreas at P3. (J-L) Amylase staining (brown) of
duct-like structures of RbpjKO pancreas at E18.5. In contrast to
ventral bud (L), the most of dorsal duct-like structures are positive for
amylase (J,K). (M-O) Amylase+ cells (green) from
RbpjKO embryos express PDX1 (M, red) and PTF1A (O, red), detected by
immunofluorescence, and are proliferating as determined by cytoplasmic amylase
(brown) and nuclear BrdU (black) staining (N, arrow). Inset in M represents a
2.6x enlargement. (P) Double-immunofluorescence staining of acini
from RbpjKO;R26R mice for amylase and β-galactosidase at P3.
(Q) PCR analysis of DNA from microdissected acinar cells of
RbpjKO (lane 1) and Rbpj+/- (lane 2) pancreata.
Schematic maps of the floxed Rbpj locus before and after Cre
recombination are shown. The position and polarity of the primers used for
amplification are represented by P8 and P9 (red arrowheads). Nuclei were
counterstained with DAPI. Amy, amylase; ac, acinar; m, mesentery. Scale bars:
50 µm (100 µm in D,E).
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