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Fig. 1. Groove markers in wild-type embryos. The figures are projections of
confocal image stacks. Unless otherwise indicated, anterior is leftwards and
dorsal is upwards. Embryos are 
500 µm long and cell diameters after
stage 11 are 4 µm. (A) Schematic representation of the groove.
Groove cells have rectangular junctions and are located at the bottom of the
groove. (B) Cross-section of a stage 14 embryo expressing β-gal
(green) in the en domain. Odd (blue) marks groove cells and Dlg (red)
reveals cell shapes. (C) Projection oriented as in A to display Crb
accumulation (green) at the subapical domain of groove cells. Cadherin, red;
Odd, blue. (D) Stage 12 wild-type embryo showing Crb (green), Odd (red)
and cadherin (blue). Grooves are absent from the ventral domain, although Crb
accumulation is visible there. (E-E''') En face view of
rectangular groove cells in a stage 14 embryo showing Ena (green; E'),
Cadherin (red; E") and aPKC (blue; E'''). Ena marks junctions
between groove cells (arrowhead). Cadherin is uniformly expressed. aPKC is
enriched in the subapical domain of groove cells. Yellow dots indicate groove
cells. (F) Stage 12 cadherin-GFP-expressing embryo showing Cadherin-GFP
(green), En (red) and Odd (blue).
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