spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Severe liver hypoplasia in vHnf1-/- embryos. (A) Direct observation of WT and vHnf1-/- mouse embryos at E13.5 shows an essentially empty abdominal cavity in the mutants (arrows). (B) Liver dissection at E14.5 reveals the liver hypoplasia of mutant embryos. (C,D) Hematoxylin and Eosin (H&E) staining of sagittal sections of WT (C) and vHnf1-/- (D) embryos at E13.5 show the reduced volume of mutant liver and the presence of hemorrhagic regions (arrow). At higher magnification, the strong disorganization of mutant liver tissue is evident (compare insets). (E,F) Immunostaining at E13.5 indicates the absence of E-cadherin expression in mutant liver (F), in contrast to WT (E) in which the staining reveals the epithelial organization of hepatoblasts. The insets illustrate correct E-cadherin expression in the stomach epithelium of WT (E) and of mutant (F) embryos. (G,H) TUNEL analysis at E13.5 shows a massive apoptosis of the majority of cells that compose the mutant liver (H), whereas in WT embryos few apoptotic cells are observed (G). The dashed line outlines the liver.





Right arrow Return to article