DEV018341 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

  Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Corresponding timing of expression of ID1, ID4 and BMP2, as well as of ID3 and BMP7. Following in situ hybridization with RNA probes, the left and right femoral tract of the same HH30 embryo were compared, the numbers correspond to the order of appearance of rows. (A,B) The second row (R2) showed five feather primordia expressing BMP2 (A), and the same number of primordia expressing ID1 was observed on the right side (B). (C,D) In another embryo, four feather primordia expressed BMP2 on the left side (C), and four primordia expressed ID4 on the right side (D). (E,F) In a third embryo, three feather primordia expressed both BMP7 on the left side (E) and ID3 on the right side (F). Scale bars: 1000 µm.

• Supplemental Figure S2 -

  Fig. S2. Dermal cell proliferation before and during dermal condensation formation in dorsal skin. DNA quantification was performed by flow cytometry on HH29 and HH30 dermal cells isolated from the medial (mp) or lateral (lp) part of the dorsal pteryla. At HH29, 83.7% of cells from the medial part were in G1 phase versus 78.9% of cells from the lateral part. The difference was increased in HH30 skin, where only 77.5% of cells from the lateral part were in G1, while 90.9% of cells were in G1 in the medial part. This difference correlated with the increase in dermal condensation rows.

• Supplemental Figure S3 -

  Fig. S3. Inhibition of spot formation by pulses of inhibitor. (A-C) An increase of local pulse concentration of inhibitor led to an increase of inhibition spot formation area, caused by the arrest of cell migration. p_v(x,y)=c*exp(−(x²+(y−0.5)²)*50), with c=20000/12, 20000/3 or 20000.

• Movie 1 -

  Movie 1. Hexagonal pattern formation in two dimensions (2D). Pattern formation resulting from the numerical resolution of the mathematical model.

• Movie 2 -

  Movie 2. Hexagonal pattern formation in three dimensions (3D). Pattern formation resulting from the numerical resolution of the mathematical model.

• Movie 3 -

  Movie 3. Peripheral inhibition led by an excess of activator effect in 2D. Pattern formation resulting from a local pulse of activator with the numerical resolution of the mathematical model. The peripheral inhibition, caused by a massive cell migration under the pulse, led to a reorganization of the hexagonal pattern.

• Movie 4 -

  Movie 4. Peripheral inhibition led by an excess of activator effect in 3D. Pattern formation resulting from the numerical resolution with a local pulse of activator of the mathematical model.