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Fig. 2. Comparative effects of BMP2 and BMP7 on dermal fibroblast
cytokinesis. A freshly dissociated dermal cell suspension was added to a
cell culture insert and basic medium placed on the other side of the 8 µm
pore membrane. Two types of control were performed: first, basic media with or
without FBS; and second, basic media with or without FBS, with FGF2. The first
control allowed a normalization of the cell migration measurement (50.1% and
100%), the second, the validation of the chemotactic effect with FGF2 (60.8%
and 110.9%). BMP7 had an even stronger chemotactic effect (70.1% and 122.3%).
Perturbation of fibroblast migration was clearly shown with BMP2 (38.1% and
81.6%). The chemotaxis obtained with BMP7 decreased with the addition of BMP2
(51.2% and 98.2%). The migration was evaluated in triplicate by a fluorescent
method. P<0.05 by Student's t-test. BMP2 and BMP7, 0.5
µg/ml; FGF2, 0.33 µg/ml.
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