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First published online July 25, 2008

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Novel aPKC regulator rises to the bait

The atypical protein kinase C (aPKC) is required for the polarisation of many cell types and has important roles in neural stem cell identity and proliferation. Yet, despite its importance for cell polarity and growth, little is known about how its activity is regulated. Now Chabu and Doe (on p. 2739) report that, in Drosophila, Dynamin-associated protein 160 (Dap160) positively regulates aPKC, and that aPKC requires it to establish neuroblast (NB) cell polarity and cell cycle progression. The authors identified Dap160 by performing immunoprecipitation experiments coupled to mass spectrometry using aPKC as the bait. They show that Dap160 directly interacts with aPKC and stimulates its activity in vitro. In vivo, it colocalises with aPKC at the apical cortex of embryonic NBs; in dap160 mutants, aPKC delocalises from here and has reduced activity. In addition, in both dap160 and aPKC mutants, fewer proliferating NBs with a prolonged cell cycle are found. Exactly how Dap160 localises aPKC and promotes cell cycle progression remain questions for the future.

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Related articles in Development:

Dap160/intersectin binds and activates aPKC to regulate cell polarity and cell cycle progression

Chiswili Chabu and Chris Q. Doe
Development 2008 135: 2739-2746. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Development Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?