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Fig. 6. Hox-negative neural crest skeletal progenitor cells assume a
Hox-positive status when placed into a Hox-positive environment.
(A,B) The adult tibial periosteum expresses Hoxa13 and
Hoxa11. (C) At post-surgical day 3, the tibial injury site is
occupied by Hoxa11-expressing cells. (D,E) By contrast,
the mandibular periosteum is devoid of Hoxa11 expression (D), and
injury does not induce Hoxa11 expression (E). (F,G)
Hox-positive tibial periosteum maintains its expression when transplanted into
a Hox-negative mandibular injury site. (H) GFP antibody staining labels
Hox-negative mandibular periosteum in a tibial injury site. (I)
Adjacent section shows Hoxa11 expression in the transplanted
mandibular periosteum (dashed line). (J,K) Skeletal progenitor
cells derived from β-actin GFP tibial periosteum express GFP (J) and
Hoxa11 (K). (L,M) When these GFP-positive, Hox-positive
cells were co-cultured with Hox-negative neural crest-derived cells (L) the
formerly Hox-negative mandibular cells begin to express Hoxa11 (M).
bm, bone marrow; ca, cartilage; mn, mandible; po, periosteum; tib, tibia.
Scale bar: 50 µm in A,B,D,F,G; 100 µm in E; 200 µm in C,H,I.
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