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Fig. 4. Fgf8 controls the size of the Rim VZ and medial Rim. Diagram shows
genes expressed around the Rim and the Stream. Electroporated brains were
collected at E12.5 and examined by two-color in situ hybridization. Only the
experimental side of the diencephalon is shown at high magnification around
ZLI (VZ is on the right side). (A-C) The Rim VZ, labeled by
Tal2 (blue), is enlarged in Fgf8-overexpressing brain (B) and
shrunken in Fgf8-inhibited brain (C) (black arrow), whereas Shh
expression (brown) is unchanged. (D-F) The medial Rim, shown by
Six3 (blue), is enlarged in Fgf8-overexpressing brain (E) and
shrunken in Fgf8-inhibited brain (F) (green arrow), while expression of
Arx in lateral diencephalon (orange arrow) is unchanged. (G-I)
The medial Rim, shown by Gad67 (blue), is specifically expanded by
Fgf8 electroporation and shrunken in Fgf8-inhibited brain (I) (pink arrow)
while expression of Pitx2-labeled cells is unchanged (blue arrow).
Scale bar in C: 125 µm.
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