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Fig. 6. Thalamic nuclei patterning is controlled by Fgf8 activity. Brains
were collected at P6 and examined with X-gal histochemistry (A), cytochrome
oxidase (CO) histochemistry (B,E,G-I) and Nissl staining (C,F). Fgf8
and Cre constructs were co-electroporated at E10.5 into R26R mice.
Brains, which have restricted staining of X-gal at the thalamus/prethalamus
(Th/PTh) border (A), exhibit a shift of the barreloid closer to the
pretectum (PTc) (B,C, bracket). Position of the mammillothalamic
tract (MMT) is indicated as a landmark. sFgfr3 and YFP
constructs were co-electroporated at E9.5. YFP fluorescence is detectable
(D), and brains exhibit slight anterior shift of the barreloid
(E,F, bracket). (G-I) Higher-magnification views of VP
show structural change in each brain. The ventral posterior medial (VPM) and
ventral posterior lateral (VPL) subfields and their position are compared with
MMT in control brain (G, double-headed arrow). Fgf8 overexpression shifts the
VPM and VPL posteriorly and compresses them (H, double-headed arrow). In
Fgf8-inhibited brain, VPM is closer to MML and VPL is stretched (l,
double-headed arrow and arrowheads). Scale bar in C: 500 µm in A-I; 200
µm in J-L.
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